Respiratory syncytial virus F expressing protein and preparation method thereof
The invention provides a respiratory syncytial virus F expressing protein and a preparation method thereof, wherein the F protein has the amino acid sequence of SEQ ID NO:1. Amino acid point mutationis carried out at a Furin recognition site of the soluble F protein; at the same time, a GCN4 fragmen...
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| creator | CHENG YUANZHENG CUI YUANPING GAO GUANG |
| description | The invention provides a respiratory syncytial virus F expressing protein and a preparation method thereof, wherein the F protein has the amino acid sequence of SEQ ID NO:1. Amino acid point mutationis carried out at a Furin recognition site of the soluble F protein; at the same time, a GCN4 fragment is added at a C end to prepare the F protein; through the sequence design, the F protein expressed in an eukaryotic system is guaranteed to avoid being enzymatically hydrolyzed by Furin so as to maintain the integrity of an extracellular fragment and the Pre-fusion state and complete immunogenicity of the F protein are maintained. The F protein is necessary to induce a body to produce antibodies with immunoprotective effect against RSV, and effectively improves the probability to screen positive antibodies in the later stage.
本发明提供了一种表达呼吸道合胞病毒F蛋白及制备方法，其中所述F蛋白的氨基酸序列为SEQ:ID:NO:1。并在可溶的F蛋白的Furin识别位点上进行氨基酸点突变；同时在C端添加了GCN4片段制备F蛋白，通过这样的序列设计，保证了在真核系统中表达出来的F蛋白避免被Furin酶解从而保持了胞外段的完整性，维持了F蛋白的Pre-fusion状态和完整的免疫原性。这样的F蛋白是诱导机体产 |
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本发明提供了一种表达呼吸道合胞病毒F蛋白及制备方法，其中所述F蛋白的氨基酸序列为SEQ:ID:NO:1。并在可溶的F蛋白的Furin识别位点上进行氨基酸点突变；同时在C端添加了GCN4片段制备F蛋白，通过这样的序列设计，保证了在真核系统中表达出来的F蛋白避免被Furin酶解从而保持了胞外段的完整性，维持了F蛋白的Pre-fusion状态和完整的免疫原性。这样的F蛋白是诱导机体产</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190430&DB=EPODOC&CC=CN&NR=109694400A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25551,76302</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190430&DB=EPODOC&CC=CN&NR=109694400A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHENG YUANZHENG</creatorcontrib><creatorcontrib>CUI YUANPING</creatorcontrib><creatorcontrib>GAO GUANG</creatorcontrib><title>Respiratory syncytial virus F expressing protein and preparation method thereof</title><description>The invention provides a respiratory syncytial virus F expressing protein and a preparation method thereof, wherein the F protein has the amino acid sequence of SEQ ID NO:1. Amino acid point mutationis carried out at a Furin recognition site of the soluble F protein; at the same time, a GCN4 fragment is added at a C end to prepare the F protein; through the sequence design, the F protein expressed in an eukaryotic system is guaranteed to avoid being enzymatically hydrolyzed by Furin so as to maintain the integrity of an extracellular fragment and the Pre-fusion state and complete immunogenicity of the F protein are maintained. The F protein is necessary to induce a body to produce antibodies with immunoprotective effect against RSV, and effectively improves the probability to screen positive antibodies in the later stage.
本发明提供了一种表达呼吸道合胞病毒F蛋白及制备方法，其中所述F蛋白的氨基酸序列为SEQ:ID:NO:1。并在可溶的F蛋白的Furin识别位点上进行氨基酸点突变；同时在C端添加了GCN4片段制备F蛋白，通过这样的序列设计，保证了在真核系统中表达出来的F蛋白避免被Furin酶解从而保持了胞外段的完整性，维持了F蛋白的Pre-fusion状态和完整的免疫原性。这样的F蛋白是诱导机体产</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjTEKwkAQRdNYiHqH8QDCikFIGYLBSkHsw5L8mIE4u-ys4t7eLTyA1X_Fe_xlcb1BPQcbXUikSfoU2c705vBSagkfH6DK8iAfXAQLWRkyw9scsRN6Ik5uoDghwI3rYjHaWbH57arYtqd7c97Buy5f2R6C2DWXvamOVVkaUx_-cb7fmjfq</recordid><startdate>20190430</startdate><enddate>20190430</enddate><creator>CHENG YUANZHENG</creator><creator>CUI YUANPING</creator><creator>GAO GUANG</creator><scope>EVB</scope></search><sort><creationdate>20190430</creationdate><title>Respiratory syncytial virus F expressing protein and preparation method thereof</title><author>CHENG YUANZHENG ; CUI YUANPING ; GAO GUANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN109694400A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CHENG YUANZHENG</creatorcontrib><creatorcontrib>CUI YUANPING</creatorcontrib><creatorcontrib>GAO GUANG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CHENG YUANZHENG</au><au>CUI YUANPING</au><au>GAO GUANG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Respiratory syncytial virus F expressing protein and preparation method thereof</title><date>2019-04-30</date><risdate>2019</risdate><abstract>The invention provides a respiratory syncytial virus F expressing protein and a preparation method thereof, wherein the F protein has the amino acid sequence of SEQ ID NO:1. Amino acid point mutationis carried out at a Furin recognition site of the soluble F protein; at the same time, a GCN4 fragment is added at a C end to prepare the F protein; through the sequence design, the F protein expressed in an eukaryotic system is guaranteed to avoid being enzymatically hydrolyzed by Furin so as to maintain the integrity of an extracellular fragment and the Pre-fusion state and complete immunogenicity of the F protein are maintained. The F protein is necessary to induce a body to produce antibodies with immunoprotective effect against RSV, and effectively improves the probability to screen positive antibodies in the later stage.
本发明提供了一种表达呼吸道合胞病毒F蛋白及制备方法，其中所述F蛋白的氨基酸序列为SEQ:ID:NO:1。并在可溶的F蛋白的Furin识别位点上进行氨基酸点突变；同时在C端添加了GCN4片段制备F蛋白，通过这样的序列设计，保证了在真核系统中表达出来的F蛋白避免被Furin酶解从而保持了胞外段的完整性，维持了F蛋白的Pre-fusion状态和完整的免疫原性。这样的F蛋白是诱导机体产</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Respiratory syncytial virus F expressing protein and preparation method thereof |
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