Respiratory syncytial virus F expressing protein and preparation method thereof

The invention provides a respiratory syncytial virus F expressing protein and a preparation method thereof, wherein the F protein has the amino acid sequence of SEQ ID NO:1. Amino acid point mutationis carried out at a Furin recognition site of the soluble F protein; at the same time, a GCN4 fragment is added at a C end to prepare the F protein; through the sequence design, the F protein expressed in an eukaryotic system is guaranteed to avoid being enzymatically hydrolyzed by Furin so as to maintain the integrity of an extracellular fragment and the Pre-fusion state and complete immunogenicity of the F protein are maintained. The F protein is necessary to induce a body to produce antibodies with immunoprotective effect against RSV, and effectively improves the probability to screen positive antibodies in the later stage. 本发明提供了一种表达呼吸道合胞病毒F蛋白及制备方法，其中所述F蛋白的氨基酸序列为SEQ:ID:NO:1。并在可溶的F蛋白的Furin识别位点上进行氨基酸点突变；同时在C端添加了GCN4片段制备F蛋白，通过这样的序列设计，保证了在真核系统中表达出来的F蛋白避免被Furin酶解从而保持了胞外段的完整性，维持了F蛋白的Pre-fusion状态和完整的免疫原性。这样的F蛋白是诱导机体产