METHODS FOR PURIFYING ANTIBODIES

METHODS FOR PURIFYING ANTIBODIES

The present invention relates to a method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the method comprising: (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography so...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: LEVY NICHOLAS E, GOKLEN KENT E, THOMSON ANDREW S, YANCEY KENNETH G, DUMETZ ANDRE C, MOLEK JESSICA RACHEL
Format: Patent
Sprache:chi ; eng
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The present invention relates to a method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the method comprising: (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer comprising caprylate and arginine; and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support. 本发明涉及从宿主细胞蛋白质(HCP)纯化重组多肽的方法，该方法包括：(a)将包含重组多肽和HCP的溶液施加至超抗原色谱固体载体；(b)用包含辛酸盐和精氨酸的洗涤缓冲液洗涤超抗原色谱固体载体；和(c)从超抗原色谱固体载体洗脱重组多肽。