Kit for detecting MCR (Mobile Colistin Resistance) gene as well as detection method and application of kit
The invention discloses a kit for detecting an MCR (Mobile Colistin Resistance) gene as well as a detection method and application of the kit. The kit comprises a PCR (Polymerase Chain Reaction)-groupA primer and a PCR-group B primer, wherein the PCR-group A primer comprises: a primer pair shown as...
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| creator | SHEN JIANZHONG LYU ZIQUAN LIU WEIWEN KE YUEBIN HU SHUANGFANG |
| description | The invention discloses a kit for detecting an MCR (Mobile Colistin Resistance) gene as well as a detection method and application of the kit. The kit comprises a PCR (Polymerase Chain Reaction)-groupA primer and a PCR-group B primer, wherein the PCR-group A primer comprises: a primer pair shown as SEQ ID No: 1 and SEQ ID No: 2, a primer pair shown as SEQ ID No: 3 and SEQ ID No: 4, a primer pairshown as SEQ ID No: 5 and SEQ ID No: 6, a primer pair shown as SEQ ID No: 7 and SEQ ID No: 8 and a primer pair shown as SEQ ID No: 9 and SEQ ID No: 10; the PCR-group B primer comprises: a primer pairshown as SEQ ID No: 11 and SEQ ID No: 12 and a primer pair shown as SEQ ID No: 13 and SEQ ID No: 14. The kit disclosed by the invention is applicable to a medical clinical or environment monitoring scene, and can be used for rapidly, conveniently and effectively carrying out detection early warning on any one of the MCR gene in an excrement sample, and carrying out real-time monitoring on a PCR product; the consumption of m |
| format | Patent |
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The kit comprises a PCR (Polymerase Chain Reaction)-groupA primer and a PCR-group B primer, wherein the PCR-group A primer comprises: a primer pair shown as SEQ ID No: 1 and SEQ ID No: 2, a primer pair shown as SEQ ID No: 3 and SEQ ID No: 4, a primer pairshown as SEQ ID No: 5 and SEQ ID No: 6, a primer pair shown as SEQ ID No: 7 and SEQ ID No: 8 and a primer pair shown as SEQ ID No: 9 and SEQ ID No: 10; the PCR-group B primer comprises: a primer pairshown as SEQ ID No: 11 and SEQ ID No: 12 and a primer pair shown as SEQ ID No: 13 and SEQ ID No: 14. The kit disclosed by the invention is applicable to a medical clinical or environment monitoring scene, and can be used for rapidly, conveniently and effectively carrying out detection early warning on any one of the MCR gene in an excrement sample, and carrying out real-time monitoring on a PCR product; the consumption of m</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190426&DB=EPODOC&CC=CN&NR=109680056A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25544,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190426&DB=EPODOC&CC=CN&NR=109680056A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHEN JIANZHONG</creatorcontrib><creatorcontrib>LYU ZIQUAN</creatorcontrib><creatorcontrib>LIU WEIWEN</creatorcontrib><creatorcontrib>KE YUEBIN</creatorcontrib><creatorcontrib>HU SHUANGFANG</creatorcontrib><title>Kit for detecting MCR (Mobile Colistin Resistance) gene as well as detection method and application of kit</title><description>The invention discloses a kit for detecting an MCR (Mobile Colistin Resistance) gene as well as a detection method and application of the kit. 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| language | chi ; eng |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Kit for detecting MCR (Mobile Colistin Resistance) gene as well as detection method and application of kit |
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