Kit for detecting MCR (Mobile Colistin Resistance) gene as well as detection method and application of kit

The invention discloses a kit for detecting an MCR (Mobile Colistin Resistance) gene as well as a detection method and application of the kit. The kit comprises a PCR (Polymerase Chain Reaction)-groupA primer and a PCR-group B primer, wherein the PCR-group A primer comprises: a primer pair shown as...

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Hauptverfasser: SHEN JIANZHONG, LYU ZIQUAN, LIU WEIWEN, KE YUEBIN, HU SHUANGFANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a kit for detecting an MCR (Mobile Colistin Resistance) gene as well as a detection method and application of the kit. The kit comprises a PCR (Polymerase Chain Reaction)-groupA primer and a PCR-group B primer, wherein the PCR-group A primer comprises: a primer pair shown as SEQ ID No: 1 and SEQ ID No: 2, a primer pair shown as SEQ ID No: 3 and SEQ ID No: 4, a primer pairshown as SEQ ID No: 5 and SEQ ID No: 6, a primer pair shown as SEQ ID No: 7 and SEQ ID No: 8 and a primer pair shown as SEQ ID No: 9 and SEQ ID No: 10; the PCR-group B primer comprises: a primer pairshown as SEQ ID No: 11 and SEQ ID No: 12 and a primer pair shown as SEQ ID No: 13 and SEQ ID No: 14. The kit disclosed by the invention is applicable to a medical clinical or environment monitoring scene, and can be used for rapidly, conveniently and effectively carrying out detection early warning on any one of the MCR gene in an excrement sample, and carrying out real-time monitoring on a PCR product; the consumption of m