Production process for increasing yield of adeno-associated viruses

The invention belongs to the field of cell engineering, and particularly relates to a production process for increasing the yield of adeno-associated viruses. The production process for increasing theyield of the adeno-associated viruses mainly comprises the steps that in the process of transfecting three plasmids of the adeno-associated viruses into 293T cells, factors promoting cell proliferation are added into a culture solution containing adherent cells, the cell proliferation is promoted, more vectors are provided for the adeno-associated viruses, three specific plasmid vectors are selected, and the in-vitro survival time of the adeno-associated viruses is prolonged. The production process for increasing the yield of the adeno-associated viruses can significantly increase the yield of the adeno-associated viruses produced by the 293T cells, the operation method is extremely simple, and a powerful basis is provided for large-scale production of the adeno-associated viruses. 本发明属于细胞工程领域，具体涉及种提高腺相关病毒产量的生产工艺