Method for detecting carbendazim and metabolites by liquid chromatogram-tandem mass spectrum

The invention, which relates to the technical field of edible mushroom detection, discloses a method for detecting carbendazim and metabolites by liquid chromatogram-tandem mass spectrum. The method comprises: mixing acetonitrile and to-be-detected sample and carrying out extraction, adding NaCl, carrying out mixing uniformly and carrying out centrifuging, carrying out purifying, and then taking supernatant to prepare sample detecting liquid; and absorbing the sample detecting liquid and injecting the liquid into a liquid chromatogram-tandem mass spectrograph to carry out detection. Therefore,the carbendazim and the metabolites like 2-aminobenzimidazole, 2-hydroxybenzimidazole and benzimidazole in the sample can be detected simultaneously. The method has advantages of high accuracy, highprecision and high sensitivity. 本发明公开了种液相色谱-串联质谱检测多菌灵及其代谢物的方法，涉及食用菌检测技术领域。该方法包括：用乙腈与待测样品混合进行提取，再加入NaCl，混匀后离心，净化后取上清液制备成样品检测液；吸取所述样品检测液注入液相色谱-串联质谱仪进行检测。该方法可以实现对样品中的多菌灵及其代谢物2-氨基苯并咪唑、2-羟基苯并咪唑和苯并咪唑同时检测。该方法具有较高的准确度、精密度和灵敏度。