Preparation method of functionalized Fe3O4/Au nano composite material and application of functionalized Fe3O4/Au nano composite material to detection of fungaltoxin

The invention provides a preparation method of a functionalized Fe3O4/Au nano composite material and application of the functionalized Fe3O4/Au nano composite material to detection of fungaltoxin. Themethod comprises the following steps that gold nanoparticles are loaded on the surfaces of magnetic beads, a DNA capturing probe arranged for marking sulfydryl at the 5' end is modified to the Fe3O4/Au surface through Au-S covalent bonds, and an ochratoxin A (OTA) aptamer chain is added to form a stable double-chain structure together with the capturing probe. The preparation method has the beneficial effects that the synthesis method of the Fe3O4/Au is simple in operation, and the Fe3O4/Au is easy to obtain; and the Fe3O4/Au composite material combines the magnetic beads to rapidly enrich ina magnetic field environment, and the advantages of the high conductivity and the good biocompatibility of the gold nanoparticles are achieved. The Fe3O4/Au combines with high selectivity of the aptamer chain, a novel sensor