Quantitative detection method of rare earth doped calcium phosphate fluorescent nanoparticles in vivo

The invention relates to a quantitative detection method of rare earth doped calcium phosphate fluorescent nanoparticles in vivo. Firstly, a rare earth ion fluorescence intensity-concentration standard curve is established, then a sample in a to-be-tested group and a sample in a blank control group are made into homogenate, supernate is taken after centrifugation to test the fluorescence intensity, fluorescence intensity values of the two parts within the unit mass or volume are calculated, and significant difference analysis is carried out; if P is larger than or equal to 0.05, it is determined that the RE-nCaP content in the sample in the to-be-tested group is 0, if P is smaller than 0.05, the tissue extraction rate of RE-nCaP is continuously tested, finally consideration is comprehensively given to the tissue extraction rate, the homogenate volume, the fluorescence intensity values within the unit mass or volume, the homogenate dilution ratio and the doping amount, and the accuratecontent of the RE-nCaP in