Method for in vitro multiplication culture of NK cells

The invention relates to a method for in vitro multiplication culture of NK cells. The method comprises coating a culture bottle with heparin sodium, CD16 and human immunoglobulin, collecting peripheral blood, inactivating plasma, separating mononuclear cells, using a serum-free medium (containing autologous plasma, IL-2, IL-15 and IL-18) to culture multiplication cells, harvesting and detecting the cells and the like. Trophoblast cells or magnetic beads are not used for sorting and purifying, and the method has simple operation, high safety, good amplification effect and high clinical application value. 本发明涉及种人NK细胞体外培养扩增的方法，包括：用肝素钠、CD16和人免疫球蛋白包被培养瓶，外周血的采集，血浆的灭活，单个核细胞的分离，用无血清培养基（含有自体血浆、IL-2、IL-15和IL-18）培养扩增细胞，细胞的收获和检测等步骤。本发明不使用滋养层细胞或磁珠分选纯化，操作简单、安全性高、扩增效果好，临床应用价值高。