Method for culturing chlorellaand increasing biomass and greaseproductive rate of chlorella

The invention discloses a method for culturing chlorella and increasing biomass and grease productive rate of the chlorella. The method comprises the following steps of (1), culturing of chlorella, wherein sodium bicarbonate is added into sterilized chlorella culturing liquid, after the chlorella is inoculated, the culturing liquid is placed in a light cultivating box for culturing for 7-10 days,mixed gas of carbon dioxide and nitrogen is introduced in the culturing liquid, the culturing temperature is controlled to be 25-28 DEG C, andillumination of white fluorescence is 100-150 micromole m-2s-1; (2), monitoring of biomass, wherein a dry weight standard curve of the chlorella is drawn, the light absorbance (OD680) of the chlorella culturing liquid at 680 nm is monitored every day in theculturing process, and the dry weight is obtained after conversion according to the standard curve; (3) grease extraction, wherein the chlorella liquid after culturing is collected, after freeze-drying, a chloroform-methanol s