Construction method and application of ultrahigh performance liquid chromatography (UPLC) fingerprint of cortex mori medicinal materials

The invention discloses a construction method and application of an ultrahigh performance liquid chromatography (UPLC) fingerprint of cortex mori medicinal materials. The construction method of the UPLC fingerprint of the cortex mori medical materials comprises the steps that a reference solution is prepared, a test solution is prepared, UPLC is determined, the fingerprint is established, similarity evaluation is carried out, and cluster analysis is conducted. According to the construction method, the method for quickly identifying the UPLC fingerprint of the cortex mori medicinal materials within 23 min is established, the similarity evaluation and the cluster analysis are conducted on the data, the construction method is simple and efficient, has good reproducibility, and provides the basis for comprehensive and effective control of and the scientific evaluation of the cortex mori medical materials. 本发明公开了种桑白皮药材UPLC指纹图谱的构建方法及其应用，包括步骤：对照品溶液的制备、供试品溶液的制备、超高相液相色谱测定、指纹图谱的建立、相似度评价和聚类分析。本发明建立了23 min内快速鉴别桑白皮药材的UPLC