Enzyme biosensor for pesticide residue detection and preparation method and application of enzyme biosensor

The invention relates to the technical field of biosensing and the field of pesticide residue detection, and discloses an enzyme biosensor for pesticide residue detection and a preparation method of the enzyme biosensor and application of the enzyme biosensor in rapid detection of organophosphorus pesticide residues. An enzyme inhibition principle is adopted, the surface of a ready-to-use type screen-printed electrode is successively modified with a graphene composite material and plant esterase to construct the enzyme biosensor. The differential-pulse voltammetry is adopted to construct a standard curve to achieve simple, rapid and highly-sensitive detection of organophosphorus pesticide residues. The adopted ready-to-use type screen-printed electrode is free of manual grinding and is simple to operate, and detection repeatability is improved. The graphene composite material with a large specific surface area and good conductivity is used for modifying the electrode, and therefore thefixed amount of the enzy