Method for rapidly screening active ingredients of drugs based on ultrafiltration-liquid chromatography-mass spectrometry

Method for rapidly screening active ingredients of drugs based on ultrafiltration-liquid chromatography-mass spectrometry

The invention discloses a method for rapidly screening active ingredients of drugs based on an ultrafiltration-liquid chromatography-mass spectrometry. The method comprises the steps of dissolving a small molecular mixture of traditional Chinese medicine extract or other sources in a buffer solution...

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Hauptverfasser: YAN BEIBEI, BAI QIAOXIU, ESON VAKAS, CHEN FUXIN, YANG QIANQIAN, YANG YAWEN, ZHOU ANNING, CHEN SUYING, GONG PIN
Format: Patent
Sprache:chi ; eng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator YAN BEIBEI
BAI QIAOXIU
ESON VAKAS
CHEN FUXIN
YANG QIANQIAN
YANG YAWEN
ZHOU ANNING
CHEN SUYING
GONG PIN
description The invention discloses a method for rapidly screening active ingredients of drugs based on an ultrafiltration-liquid chromatography-mass spectrometry. The method comprises the steps of dissolving a small molecular mixture of traditional Chinese medicine extract or other sources in a buffer solution, and adding enzyme for co-incubation; filtering the solution by ultracentrifugation concentration,repeating the filtering for multiple times, merging concentrated solutions, and making up the volume by the buffer solution; and analyzing the change of a chromatographic peak after the co-incubationof the enzyme by adopting a liquid chromatography-mass spectrometry technology. The method disclosed by the invention has the advantages that the hypoglycemic activity ingredients of the traditional Chinese medicines are screened by virtue of the ultrafiltration-UPLC-MS; and an experiment result shows that the method can carry out preliminary rapid screening on active small molecule compounds of anatural source in the comp
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Method for rapidly screening active ingredients of drugs based on ultrafiltration-liquid chromatography-mass spectrometry
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