Method for rapidly screening active ingredients of drugs based on ultrafiltration-liquid chromatography-mass spectrometry

The invention discloses a method for rapidly screening active ingredients of drugs based on an ultrafiltration-liquid chromatography-mass spectrometry. The method comprises the steps of dissolving a small molecular mixture of traditional Chinese medicine extract or other sources in a buffer solution, and adding enzyme for co-incubation; filtering the solution by ultracentrifugation concentration,repeating the filtering for multiple times, merging concentrated solutions, and making up the volume by the buffer solution; and analyzing the change of a chromatographic peak after the co-incubationof the enzyme by adopting a liquid chromatography-mass spectrometry technology. The method disclosed by the invention has the advantages that the hypoglycemic activity ingredients of the traditional Chinese medicines are screened by virtue of the ultrafiltration-UPLC-MS; and an experiment result shows that the method can carry out preliminary rapid screening on active small molecule compounds of anatural source in the comp