PCR primer, method and kit for detecting traditional Chinese medical material contaminant aspergillus versicolor producing strain

The invention discloses a PCR primer, method and kit for detecting traditional Chinese medical material contaminant aspergillus versicolor toxic fungi. On the basis of a PCR detection method, a primerpair capable of performing PCR reaction in a reaction tube is used. A PCR system for directly detect...

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Hauptverfasser:	LUO CHAOQUAN, WANG WENLI, XU HUI, CHEN WEIWEN, LU XIAOFANG, ZHENG RUNSHENG, ZHAN RUOTING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a PCR primer, method and kit for detecting traditional Chinese medical material contaminant aspergillus versicolor toxic fungi. On the basis of a PCR detection method, a primerpair capable of performing PCR reaction in a reaction tube is used. A PCR system for directly detecting the traditional Chinese medical material contaminant aspergillus versicolor toxic fungi withoutseparation or purification is built by the invention; the specificity of each primer is high; the detection limit of the method is 101 to 102 CUF/g; the detection sensitivity is high. 本发明公开了种检测中药材污染杂色曲霉毒素产毒真菌的PCR引物、方法及试剂盒。基于PCR检测方法，包括能在反应管中进行PCR反应的引物对。本发明建立了种不经过分离纯化，直接检测中药材污染杂色曲霉毒素产毒真菌的PCR体系，各引物特异性强，方法的检出限为10～10CFU/g，检测灵敏度高。