DNA PROBES FOR IN SITU HYBRIDIZATION ON CHROMOSOMES

The invention relates to a kit, to probe mixtures, and to probes for detecting a chromosomal abnormality. A gene probe, obtained by means of a method having the following steps: (a) examining genomicsections for sequence ranges having non-repetitive nucleic acid sequences and selecting one or more nucleic acid sequences; (b) designing and synthesizing primer pairs for a polymerase chain reactionon the non-repetitive nucleic acid sequences, wherein the primers each have an oligonucleotide sequence complementary to the strand or antisense strand of the non-repetitive nucleic acid sequence, anda non-complementary universal linker sequence; (c) performing a first PCR and obtaining a first mixture (Pool A) of nucleic acid fragments; (d) performing a multiplex PCR on the mixture (Pool A) by using primers that hybridize to the linkers and obtaining a mixture (Pool B) having reproduced non-repetitive nucleic acid fragments that are suitable for chromogenic or fluorescent in situ hybridization of chromosomes (FISH/CIS