MBTH-like proteins in eukaryotic NRPS-catalyzed processes

The present invention relates to a method to improve the production of a secondary metabolite catalyzed by a non-ribosomal peptide synthetase comprising contacting in a eukaryotic host a eukaryotic non-ribosomal peptide synthetase with an MbtH-like protein. The present invention further relates to a...

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Bibliographische Detailangaben
Hauptverfasser:	BOVENBERG ROELOF ARY LANS, MULLER ULRIKE MARIA, CARSTEN POHL, DRIESSEN ARNOLD JACOB MATHIEU, ZWAHLEN RETO DANIEL
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method to improve the production of a secondary metabolite catalyzed by a non-ribosomal peptide synthetase comprising contacting in a eukaryotic host a eukaryotic non-ribosomal peptide synthetase with an MbtH-like protein. The present invention further relates to a composition comprising a eukaryotic non-ribosomal peptide synthetase that is not a hybrid and a prokaryotic MbtH and to a eukaryotic host cell comprising a non-ribosomal peptide synthetase and a polynucleotide allowing the expression of an MbtH-like protein. 本发明涉及改善由非核糖体肽合成酶催化的次生代谢物的产生的方法，该方法包括在真核宿主中使真核非核糖体肽合成酶和MbtH样蛋白接触。本发明进步涉及包含作为非杂合体的真核非核糖体肽合成酶和原核MbtH的组合物，以及包含非核糖体肽合成酶和允许表达MbtH样蛋白的多核苷酸的真核宿主细胞。