Tissue culture method of Clerodendrum inerme

A tissue culture method of Clerodendrum inerme includes the steps of disinfecting an explant, wherein the explant is a semi-lignified twig isolated from a Clerodendrum inerme seedling; cutting the explant into stem segments 1.0-2.5 cm in length, wherein each stem segment carries one bud point; inoculating the stem segments to an induced culture medium to carry out induced culture for 12-45 days toobtain cluster buds; dividing the cluster buds into a plurality of plantlets, and inoculating the plantlets to an enrichment medium to carry out enrichment culture for 38-45 days to obtain secondarybuds; cutting the secondary buds into single-bud stem segments with a top bud; inoculating the single-bud stem segments to a rooting medium to carry out rooting culture to obtain rooted seedlings. Thetissue culture method of Clerodendrum inerme has the advantages that massive seedlings can be attained quickly, tissue culture is not limited to seasons, the production requirement on its demand canbe met, seedling growth cost