Method for culturing suspended cell virus capable of maintaining production stability of virus antigens and improving effective content of viruses

The invention provides a method for culturing a suspended cell virus capable of maintaining the production stability of virus antigens and improving the effective content of viruses. The method uses aserum-free culture method in a cell culture stage, and the first-time medium change reduces the influence of cell metabolites on cell growth and reduces the interference on late-stage virus production. After the medium change, the added dextran sulfate effectively plays the effects of improving virus infection sensitivity and cell dispersion. PEG 6000 and a high-calcium solution are added to a later-phase virus inoculation and medium change system so as to improve the virus inoculation efficiency and to greatly increase the stability of foot-and-mouth disease virus. The titer of the preparedvirus solution can reach 107.0-109.01 ogTCID50/mL, the content of complete antigen 146S can reach 6 [mu]g/ml or above, and the cultured foot-and-mouth disease virus is more stable. 本发明提供了种维持病毒抗原生产稳定性，提高病毒有效含量的悬浮细胞病毒的培养方法，该方法在细