Extraction method and application of total coumarins substances in Indian stringbush roots

The invention discloses an extraction method of total coumarins substances in Indian stringbush roots. The method mainly comprises the following steps of extracting Indian stringbush root coarse powder by ethanol under ultrasound conditions; concentrating the ethanol extraction liquid; then performing dilution by water; performing extraction sequentially by ethyl acetate and normal butanol; merging extracts; performing mixed suspension by water; performing purification by macroporous absorbent resin; performing elution sequentially by 55-percent ethanol and elution liquid prepared from chloroform and methanol according to the volume ratio of 1:1; merging the elution liquid; performing concentration and drying to obtain the total coumarins substances. The extraction method has the advantages that the extraction rate of the obtained total coumarins substances is high; the extraction speed is high; the method is suitable for industrialized production; the extracted total coumarins substances have low toxic and s