Kit and method for identifying culicoides actoni

The invention discloses a kit for identifying culicoides actoni and a using method of the kit. The kit comprises a first primer group capable of amplifying CO I genes and a second primer group capableof amplifying ITS2 genes, wherein the first primer group comprises a first upstream primer with a nu...

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Bibliographische Detailangaben
Hauptverfasser:	YAO FEIRONG, LIAN GUOSHENG, LIU XIAOHUI, WANG SHENGQI, SHI SHUNLI, CI YING, PANG LIANPING, LI YUN, WANG DEWEI, SHI LINGLI, LAN JING, YAN JIHUAN, FU XIAOYUN, HUANG ENJIONG, SHEN JUN, LI WEI, GAO ZHIYUN, ZHANG MIAO, TIAN JIE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a kit for identifying culicoides actoni and a using method of the kit. The kit comprises a first primer group capable of amplifying CO I genes and a second primer group capableof amplifying ITS2 genes, wherein the first primer group comprises a first upstream primer with a nucleotide sequence shown in SEQ ID NO:1 and a first downstream primer with a nucleotide sequence shown in SEQ ID NO:2, and the second primer group comprises a second upstream primer with a nucleotide sequence shown in SEQ ID NO:4 and a second downstream primer with a nucleotide sequence shown in SEQID NO:5. The culicoides actoni is identified through a method that two different molecular markers are combined, the culicoides actoni is accurately identified advantageously, and the identificationtime is shortened. 本发明公开了种鉴定琉球库蠓的试剂盒及其使用方法，该试剂盒包括能够扩增CO I基因的第引物组和能够扩增ITS2基因的第二引物组，其中，第引物组包括具有SEQ ID NO:1所示核苷酸序列的第上游引物和具有SEQ ID NO:2所示核苷酸序列的第下游引物，第二引物组包括具有SEQ ID NO:4所示核苷酸序列的第二上游引物和具有SEQ ID NO:5所示核苷酸序列的第二下游引物。本发明采用两种不同分子标记相结合的方法