Preparation of typhoid bacillus recombinant protein and monoclonal antibodies thereof

The invention relates to the field of biotechnology and discloses preparation of a typhoid bacillus recombinant protein and monoclonal antibodies thereof. The typhoid bacillus recombinant protein disclosed by the invention has an amino acid sequence as shown in SEQ ID No:1. In order to enhance the expression effect of the recombinant protein in prokaryotic cells, a section of flexible fragments (four glycines) are applied as linkage short peptides, sequences of the dominant epitope A and the dominant epitope B of a typhoid bacillus flagella (H) antigen are connected in series and then repeatedthree times to form the recombinant protein. The invention further relates to preparation of the recombinant protein monoclonal antibodies. A monoclonal cell strain is obtained by immunization, cellfusion and multiple rounds of screening, the monoclonal antibodies are purified and respectively labeled by fluorescent microspheres, and the best monoclonal antibody pairing combination is determinedby an orthogonal experimen