Coxsackie virus attenuated strain and application thereof

The invention provides a coxsackie virus attenuated strain and application thereof. The coxsackie virus attenuated strain is Enterovirus coxsackie virus CVB5 JZ-P50 with the preservation number beingCCTCC No. V201858. The coxsackie virus B5 strain is attenuated in an attenuated live vaccine mode. Ve...

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Hauptverfasser:	YUAN RONGLIANG, SUN WEI, YANG JUNYU, ZHANG QIUSHI, LIN XIANRONG, CHEN RUIQI, CHEN JIANMIN, LUAN XIAODAN, SHAO CONGWEN, CHEN WEIDONG, ZHANG JUAN, YIN SHAOYUN, WANG RUIBO, JING MINMIN, ZHANG YEJIAO, KE JIANGWEI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a coxsackie virus attenuated strain and application thereof. The coxsackie virus attenuated strain is Enterovirus coxsackie virus CVB5 JZ-P50 with the preservation number beingCCTCC No. V201858. The coxsackie virus B5 strain is attenuated in an attenuated live vaccine mode. Vero cells are used and continuously cultured for 50 passages at a low temperature, and a CVB5 attenuated vaccine is accidentally obtained. Compared with wild virus strains, the coxsackie virus attenuated strain has the advantageous characteristics of cold adaptation and temperature sensibility, andis improved in properties of in-vivo attenuation property, immunogenicity and genetic safety, thus providing guarantee in reducing the production cost and preparing the effective, safe and cheap CVB5vaccines. 本发明提供了种柯萨奇病毒减毒株及其应用，所述的柯萨奇病毒减毒株为柯萨奇病毒B组5型JZ-P50株（Enterovirus coxsackie virus CVB5 JZ-P50），保藏编号为CCTCC No：V201858。本发明利用减毒活疫苗的方式，对柯萨奇病毒B5毒株进行减毒，所用细胞为Vero细胞，通过低温连续培养50代，意外地获得CVB5减毒疫苗，与野生病毒株相比，具有冷适应性和温度敏感性的优势特征，具有体内减毒特性、免疫