Double-antigen sandwich antibody detection method

The invention relates to a double-antigen sandwich antibody detection method. According to the method, detection is finished in a manner of forming a solid-phase support-a first antigen Ag1-a to-be-detected antibody-a second antigen Ag2, wherein the Ag2 is coupled with a marker for displaying signal strength; the method comprises the following steps: 1), enabling the Ag1 and the Ag2 to be in contact with a to-be-detected object under the condition that antigen/antibody reaction can occur with enough Ag1, Ag2 and the to-be-detected object, so as to form an immune compound, wherein the content of the Ag1 is greater than that of the Ag2 based on mol number; 2) washing the unbound to-be-detected antibody; 3) adding the Ag2 to bind the Ag2 with residual antigen binding sites in the immune compound; 4) detecting the markers to indicate the existence and/or content of the to-be-detected antibody. Compared with the prior art, the method provided by the invention ensures the low-value sensitivity of detection, also ca