Real-time fluorescence RT-PCR detection kit for bee IAPV (Israeli acute paralysis virus) and detection method of detection kit

The invention relates to a real-time fluorescence RT-PCR detection kit for a bee IAPV (Israeli acute paralysis virus) and a detection method of the detection kit. The detection kit is specially used for detecting the bee IAPV. The kit comprises (1) an RT-PCR reaction solution, (2) Taq DNA polymerase, (3) reverse transcriptase, (4) positive standard, (5) negative control and (6) sterile water. Thedetection kit has the following advantages: (1), good stability and specificity: detection of the bee IAPV has high specificity, cross reaction with other bee viruses is avoided, and repeatability isgood; (2), high sensitivity: the sensitivity can reach 10 copies/mu L; (3), convenience and rapidness in operation: the whole reaction can be completed within 2 hours. The kit can be used for detection of the bee IAPV and differential diagnosis of the bee IAPV with other bee virus disease, and has great significance for early prevention and timely treatment of the disease. 本发明涉及种蜜蜂以色列急性麻痹病毒实时荧光RT-PCR检测试剂盒及其检测方法，专用于蜜蜂以色列急性麻