Multiple PCR detection method of swine pathogens
The invention discloses a multiple PCR detection method of swine pathogens. The method includes steps of to-be-detected sample DNA extraction, PCR, PCR amplification, amplification product electrophoresis detection and electrophoresis detection result analysis. A system of PCR comprises 2.5uL of Buf...
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| creator | ZHANG HUAQI GUI GANBEI QI SHIJIN ZENG ZE |
| description | The invention discloses a multiple PCR detection method of swine pathogens. The method includes steps of to-be-detected sample DNA extraction, PCR, PCR amplification, amplification product electrophoresis detection and electrophoresis detection result analysis. A system of PCR comprises 2.5uL of Buffer, 1.5uL of Mg2+, 2uL of dNTP, 0.5uL of SS upstream primer, 0.5uL of downstream primer, 0.5uL of HPS upstream primer, 0.5uL of HPS downstream primer, 0.5uL of Mph upstream primer, 0.5uL of Mph downstream primer, 0.25uL of Taq enzyme, 2uL of template and the balance of sterile deionized water up to25uL. Conventional detection methods of the swine pathogens are relatively time-consuming and labor-consuming, thereby being bad for efficient detection; on the basis of an objective of quickly and accurately diagnosing mixed infectious pathogens, various advantages of multiple PCR detection are utilized to establish a quick, specific and sensitive multiple PCR diagnosis method of the swine pathogens, and quick, accurate |
| format | Patent |
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The method includes steps of to-be-detected sample DNA extraction, PCR, PCR amplification, amplification product electrophoresis detection and electrophoresis detection result analysis. A system of PCR comprises 2.5uL of Buffer, 1.5uL of Mg2+, 2uL of dNTP, 0.5uL of SS upstream primer, 0.5uL of downstream primer, 0.5uL of HPS upstream primer, 0.5uL of HPS downstream primer, 0.5uL of Mph upstream primer, 0.5uL of Mph downstream primer, 0.25uL of Taq enzyme, 2uL of template and the balance of sterile deionized water up to25uL. Conventional detection methods of the swine pathogens are relatively time-consuming and labor-consuming, thereby being bad for efficient detection; on the basis of an objective of quickly and accurately diagnosing mixed infectious pathogens, various advantages of multiple PCR detection are utilized to establish a quick, specific and sensitive multiple PCR diagnosis method of the swine pathogens, and quick, accurate</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190308&DB=EPODOC&CC=CN&NR=109439775A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190308&DB=EPODOC&CC=CN&NR=109439775A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG HUAQI</creatorcontrib><creatorcontrib>GUI GANBEI</creatorcontrib><creatorcontrib>QI SHIJIN</creatorcontrib><creatorcontrib>ZENG ZE</creatorcontrib><title>Multiple PCR detection method of swine pathogens</title><description>The invention discloses a multiple PCR detection method of swine pathogens. The method includes steps of to-be-detected sample DNA extraction, PCR, PCR amplification, amplification product electrophoresis detection and electrophoresis detection result analysis. A system of PCR comprises 2.5uL of Buffer, 1.5uL of Mg2+, 2uL of dNTP, 0.5uL of SS upstream primer, 0.5uL of downstream primer, 0.5uL of HPS upstream primer, 0.5uL of HPS downstream primer, 0.5uL of Mph upstream primer, 0.5uL of Mph downstream primer, 0.25uL of Taq enzyme, 2uL of template and the balance of sterile deionized water up to25uL. 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The method includes steps of to-be-detected sample DNA extraction, PCR, PCR amplification, amplification product electrophoresis detection and electrophoresis detection result analysis. A system of PCR comprises 2.5uL of Buffer, 1.5uL of Mg2+, 2uL of dNTP, 0.5uL of SS upstream primer, 0.5uL of downstream primer, 0.5uL of HPS upstream primer, 0.5uL of HPS downstream primer, 0.5uL of Mph upstream primer, 0.5uL of Mph downstream primer, 0.25uL of Taq enzyme, 2uL of template and the balance of sterile deionized water up to25uL. Conventional detection methods of the swine pathogens are relatively time-consuming and labor-consuming, thereby being bad for efficient detection; on the basis of an objective of quickly and accurately diagnosing mixed infectious pathogens, various advantages of multiple PCR detection are utilized to establish a quick, specific and sensitive multiple PCR diagnosis method of the swine pathogens, and quick, accurate</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Multiple PCR detection method of swine pathogens |
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