Multiple PCR detection method of swine pathogens

The invention discloses a multiple PCR detection method of swine pathogens. The method includes steps of to-be-detected sample DNA extraction, PCR, PCR amplification, amplification product electrophoresis detection and electrophoresis detection result analysis. A system of PCR comprises 2.5uL of Buffer, 1.5uL of Mg2+, 2uL of dNTP, 0.5uL of SS upstream primer, 0.5uL of downstream primer, 0.5uL of HPS upstream primer, 0.5uL of HPS downstream primer, 0.5uL of Mph upstream primer, 0.5uL of Mph downstream primer, 0.25uL of Taq enzyme, 2uL of template and the balance of sterile deionized water up to25uL. Conventional detection methods of the swine pathogens are relatively time-consuming and labor-consuming, thereby being bad for efficient detection; on the basis of an objective of quickly and accurately diagnosing mixed infectious pathogens, various advantages of multiple PCR detection are utilized to establish a quick, specific and sensitive multiple PCR diagnosis method of the swine pathogens, and quick, accurate