Recombinant attenuated salmonella LH430, as well as preparation method and use thereof
The invention discloses a recombinant attenuated salmonella LH430, as well as a preparation method and use thereof. A target gene is cloned to a pMD-18-T vector by using an MEL (Melittin) gene and anRGD (Tumor-Targeted Peptide) gene; after a shuttle vector is linearly recombined, a recombinant eukar...
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| creator | SHANG CUILING JIN TIANMING MENG JIALI GAO KEKE LI XIAOHUI LI XIN LIN JING WANG XIAOYUE LAN ZHAOYU LI DAN |
| description | The invention discloses a recombinant attenuated salmonella LH430, as well as a preparation method and use thereof. A target gene is cloned to a pMD-18-T vector by using an MEL (Melittin) gene and anRGD (Tumor-Targeted Peptide) gene; after a shuttle vector is linearly recombined, a recombinant eukaryotic expression vector pEGFP-RGD-MEL is constructed and is transformed into a competent LH430, andthe recombinant attenuated salmonella LH430 containing the abovementioned target gene is constructed; and a melanoma cell B16 is infected by using the recombinant attenuated salmonella LH430, so thatthe melanoma cell B16 expresses the RGD-MEL gene. The tumor suppression effect of the RGD-MEL gene co-expressed attenuated salmonella LH430 on the B16 cell is evaluated.
本发明公开了种重组减毒沙门氏菌LH430及其制备方法和用途，利用蜂毒肽MEL基因和肿瘤靶向肽RGD基因，将目的基因克隆到pMD-18-T载体中，线性化重组穿梭载体后构建重组真核表达载体pEGFP-RGD-MEL，将其转化至感受态LH430，构建了包含上述目的基因的重组减毒沙门氏菌LH430；用重组减毒沙门氏菌LH430侵染黑色素瘤细胞B16，使其表达RGD-MEL基因；评价RGD-MEL基因共表达的减毒沙门氏菌LH430对B16细胞的抑瘤效果。 |
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本发明公开了种重组减毒沙门氏菌LH430及其制备方法和用途，利用蜂毒肽MEL基因和肿瘤靶向肽RGD基因，将目的基因克隆到pMD-18-T载体中，线性化重组穿梭载体后构建重组真核表达载体pEGFP-RGD-MEL，将其转化至感受态LH430，构建了包含上述目的基因的重组减毒沙门氏菌LH430；用重组减毒沙门氏菌LH430侵染黑色素瘤细胞B16，使其表达RGD-MEL基因；评价RGD-MEL基因共表达的减毒沙门氏菌LH430对B16细胞的抑瘤效果。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190308&DB=EPODOC&CC=CN&NR=109439686A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25551,76304</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190308&DB=EPODOC&CC=CN&NR=109439686A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHANG CUILING</creatorcontrib><creatorcontrib>JIN TIANMING</creatorcontrib><creatorcontrib>MENG JIALI</creatorcontrib><creatorcontrib>GAO KEKE</creatorcontrib><creatorcontrib>LI XIAOHUI</creatorcontrib><creatorcontrib>LI XIN</creatorcontrib><creatorcontrib>LIN JING</creatorcontrib><creatorcontrib>WANG XIAOYUE</creatorcontrib><creatorcontrib>LAN ZHAOYU</creatorcontrib><creatorcontrib>LI DAN</creatorcontrib><title>Recombinant attenuated salmonella LH430, as well as preparation method and use thereof</title><description>The invention discloses a recombinant attenuated salmonella LH430, as well as a preparation method and use thereof. A target gene is cloned to a pMD-18-T vector by using an MEL (Melittin) gene and anRGD (Tumor-Targeted Peptide) gene; after a shuttle vector is linearly recombined, a recombinant eukaryotic expression vector pEGFP-RGD-MEL is constructed and is transformed into a competent LH430, andthe recombinant attenuated salmonella LH430 containing the abovementioned target gene is constructed; and a melanoma cell B16 is infected by using the recombinant attenuated salmonella LH430, so thatthe melanoma cell B16 expresses the RGD-MEL gene. The tumor suppression effect of the RGD-MEL gene co-expressed attenuated salmonella LH430 on the B16 cell is evaluated.
本发明公开了种重组减毒沙门氏菌LH430及其制备方法和用途，利用蜂毒肽MEL基因和肿瘤靶向肽RGD基因，将目的基因克隆到pMD-18-T载体中，线性化重组穿梭载体后构建重组真核表达载体pEGFP-RGD-MEL，将其转化至感受态LH430，构建了包含上述目的基因的重组减毒沙门氏菌LH430；用重组减毒沙门氏菌LH430侵染黑色素瘤细胞B16，使其表达RGD-MEL基因；评价RGD-MEL基因共表达的减毒沙门氏菌LH430对B16细胞的抑瘤效果。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNysEKgkAURuHZtIjqHW77AmNEdBlSuIgWEW3lpr8ojHcG50qvX0EP0OrjwFmaxw2NH5-DsCixKmRmRUuR3egFzjFdqtQmO-JIr09_DRMCT6yDFxqhvW-JpaU5grTHBN-tzaJjF7H5uTLb8-leVnsEXyMGbiDQurwekiK1RZZnR_vP8wazqDjI</recordid><startdate>20190308</startdate><enddate>20190308</enddate><creator>SHANG CUILING</creator><creator>JIN TIANMING</creator><creator>MENG JIALI</creator><creator>GAO KEKE</creator><creator>LI XIAOHUI</creator><creator>LI XIN</creator><creator>LIN JING</creator><creator>WANG XIAOYUE</creator><creator>LAN ZHAOYU</creator><creator>LI DAN</creator><scope>EVB</scope></search><sort><creationdate>20190308</creationdate><title>Recombinant attenuated salmonella LH430, as well as preparation method and use thereof</title><author>SHANG CUILING ; JIN TIANMING ; MENG JIALI ; GAO KEKE ; LI XIAOHUI ; LI XIN ; LIN JING ; WANG XIAOYUE ; LAN ZHAOYU ; LI DAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN109439686A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SHANG CUILING</creatorcontrib><creatorcontrib>JIN TIANMING</creatorcontrib><creatorcontrib>MENG JIALI</creatorcontrib><creatorcontrib>GAO KEKE</creatorcontrib><creatorcontrib>LI XIAOHUI</creatorcontrib><creatorcontrib>LI XIN</creatorcontrib><creatorcontrib>LIN JING</creatorcontrib><creatorcontrib>WANG XIAOYUE</creatorcontrib><creatorcontrib>LAN ZHAOYU</creatorcontrib><creatorcontrib>LI DAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SHANG CUILING</au><au>JIN TIANMING</au><au>MENG JIALI</au><au>GAO KEKE</au><au>LI XIAOHUI</au><au>LI XIN</au><au>LIN JING</au><au>WANG XIAOYUE</au><au>LAN ZHAOYU</au><au>LI DAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Recombinant attenuated salmonella LH430, as well as preparation method and use thereof</title><date>2019-03-08</date><risdate>2019</risdate><abstract>The invention discloses a recombinant attenuated salmonella LH430, as well as a preparation method and use thereof. A target gene is cloned to a pMD-18-T vector by using an MEL (Melittin) gene and anRGD (Tumor-Targeted Peptide) gene; after a shuttle vector is linearly recombined, a recombinant eukaryotic expression vector pEGFP-RGD-MEL is constructed and is transformed into a competent LH430, andthe recombinant attenuated salmonella LH430 containing the abovementioned target gene is constructed; and a melanoma cell B16 is infected by using the recombinant attenuated salmonella LH430, so thatthe melanoma cell B16 expresses the RGD-MEL gene. The tumor suppression effect of the RGD-MEL gene co-expressed attenuated salmonella LH430 on the B16 cell is evaluated.
本发明公开了种重组减毒沙门氏菌LH430及其制备方法和用途，利用蜂毒肽MEL基因和肿瘤靶向肽RGD基因，将目的基因克隆到pMD-18-T载体中，线性化重组穿梭载体后构建重组真核表达载体pEGFP-RGD-MEL，将其转化至感受态LH430，构建了包含上述目的基因的重组减毒沙门氏菌LH430；用重组减毒沙门氏菌LH430侵染黑色素瘤细胞B16，使其表达RGD-MEL基因；评价RGD-MEL基因共表达的减毒沙门氏菌LH430对B16细胞的抑瘤效果。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Recombinant attenuated salmonella LH430, as well as preparation method and use thereof |
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