Recombinant attenuated salmonella LH430, as well as preparation method and use thereof

The invention discloses a recombinant attenuated salmonella LH430, as well as a preparation method and use thereof. A target gene is cloned to a pMD-18-T vector by using an MEL (Melittin) gene and anRGD (Tumor-Targeted Peptide) gene; after a shuttle vector is linearly recombined, a recombinant eukaryotic expression vector pEGFP-RGD-MEL is constructed and is transformed into a competent LH430, andthe recombinant attenuated salmonella LH430 containing the abovementioned target gene is constructed; and a melanoma cell B16 is infected by using the recombinant attenuated salmonella LH430, so thatthe melanoma cell B16 expresses the RGD-MEL gene. The tumor suppression effect of the RGD-MEL gene co-expressed attenuated salmonella LH430 on the B16 cell is evaluated. 本发明公开了种重组减毒沙门氏菌LH430及其制备方法和用途，利用蜂毒肽MEL基因和肿瘤靶向肽RGD基因，将目的基因克隆到pMD-18-T载体中，线性化重组穿梭载体后构建重组真核表达载体pEGFP-RGD-MEL，将其转化至感受态LH430，构建了包含上述目的基因的重组减毒沙门氏菌LH430；用重组减毒沙门氏菌LH430侵染黑色素瘤细胞B16，使其表达RGD-MEL基因；评价RGD-MEL基因共表达的减毒沙门氏菌LH430对B16细胞的抑瘤效果。