Primer set, kit and method for detecting two or more target nucleic acids
Provided is an improved means for detecting two or more target nucleic acids. The primer set according to the present invention comprises: terminal primer A which contains a polynucleotide containinga base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'-termin...
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| creator | NISHIZONO TAKASHI MIYAMOTO SHIGEHIKO |
| description | Provided is an improved means for detecting two or more target nucleic acids. The primer set according to the present invention comprises: terminal primer A which contains a polynucleotide containinga base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'-terminus of a complementary sequence of a first target nucleic acid sequence; a k-headed primer which contains twopolynucleotides bound together in the 5'-terminal side, wherein one of the two polynucleotides contains a base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'-terminus ofthe base sequence of a k-th target nucleic acid, and the other of the two polynucleotides contains a base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'-terminus of a complementary sequence of the base sequence of a (k+1)-th target nucleic acid; and terminal primer B which contains a polynucleotide containing a base sequence at the 3'-terminus, the base sequence being hybridizable with the 3' |
| format | Patent |
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The primer set according to the present invention comprises: terminal primer A which contains a polynucleotide containinga base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'-terminus of a complementary sequence of a first target nucleic acid sequence; a k-headed primer which contains twopolynucleotides bound together in the 5'-terminal side, wherein one of the two polynucleotides contains a base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'-terminus ofthe base sequence of a k-th target nucleic acid, and the other of the two polynucleotides contains a base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'-terminus of a complementary sequence of the base sequence of a (k+1)-th target nucleic acid; and terminal primer B which contains a polynucleotide containing a base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190301&DB=EPODOC&CC=CN&NR=109415721A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190301&DB=EPODOC&CC=CN&NR=109415721A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>NISHIZONO TAKASHI</creatorcontrib><creatorcontrib>MIYAMOTO SHIGEHIKO</creatorcontrib><title>Primer set, kit and method for detecting two or more target nucleic acids</title><description>Provided is an improved means for detecting two or more target nucleic acids. The primer set according to the present invention comprises: terminal primer A which contains a polynucleotide containinga base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'-terminus of a complementary sequence of a first target nucleic acid sequence; a k-headed primer which contains twopolynucleotides bound together in the 5'-terminal side, wherein one of the two polynucleotides contains a base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'-terminus ofthe base sequence of a k-th target nucleic acid, and the other of the two polynucleotides contains a base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'-terminus of a complementary sequence of the base sequence of a (k+1)-th target nucleic acid; and terminal primer B which contains a polynucleotide containing a base sequence at the 3'-terminus, the base sequence being hybridizable with the 3'</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEKwjAYReEsDqK-w3VXMGoRRymKLuLgXkJyW4NNUpJffH0dfACnAx9nrC637AMzCmWBpxeY6BAoj-TQpgxHoRUfO8g74QshZUJM7iiIL9vTWxjrXZmqUWv6wtmvEzU_He_1eckhNSyDsYyUpr7q1X6rq91aHzb_PB97QzPp</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>NISHIZONO TAKASHI</creator><creator>MIYAMOTO SHIGEHIKO</creator><scope>EVB</scope></search><sort><creationdate>20190301</creationdate><title>Primer set, kit and method for detecting two or more target nucleic acids</title><author>NISHIZONO TAKASHI ; MIYAMOTO SHIGEHIKO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN109415721A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>NISHIZONO TAKASHI</creatorcontrib><creatorcontrib>MIYAMOTO SHIGEHIKO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>NISHIZONO TAKASHI</au><au>MIYAMOTO SHIGEHIKO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Primer set, kit and method for detecting two or more target nucleic acids</title><date>2019-03-01</date><risdate>2019</risdate><abstract>Provided is an improved means for detecting two or more target nucleic acids. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Primer set, kit and method for detecting two or more target nucleic acids |
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