Kit for detecting human matrix metalloproteinase1 by one-step real-time fluorescence quantitative reverse transcription polymerase chain reaction

The invention relates to a kit for detecting human matrix metalloproteinase 1 by real-time fluorescence quantitative RT-PCR. The kit uses mRNA as a template to carry out reverse transcription and real-time fluorescent quantitative PCR in a same reaction system without opening a tube cover in the reaction process, so that cross contamination is avoided and the mRNA expression of the human matrix metalloproteinase 1 (MMP1) in a specimen can be accurately quantified. The kit can be used for detecting the expression of MMP1 in resected specimens in colorectal cancer surgery in clinical and scientific research, and is used for assisting the diagnosis of patients with colorectal cancer and guiding the treatment of liver metastasis. 本发明为种实时荧光定量RT-PCR检测人基质金属蛋白酶1的试剂盒，该试剂盒以mRNA为模板，使反转录和实时荧光定量PCR在同反应体系中进行，反应过程中无需打开管盖，避免了交叉污染，并可以精确定量检测标本中人基质金属蛋白酶1 (MMP1) 的mRNA表达量。该试剂盒可以用于临床及科研中检测患者结直肠癌手术切除标本中MMP1的表达，用以结直肠癌患者辅助诊断、指导治疗是否存在肝转移的指标。