Construction method and detection method of HPLC feature map of golden cypress medicinal material

The invention relates to a construction method and a detection method of a HPLC feature map of a golden cypress medicinal material. The construction method comprises the following steps: reference solution preparation: preparing a golden cypress control medicinal material reference solution and a be...

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Bibliographische Detailangaben
Hauptverfasser: WEI MEI, CHEN XIANGDONG, HUANG XIAODAN, CHENG XUEREN, HUO WENJIE, WANG MINYU, CHEN WANFA, PAN LIYE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a construction method and a detection method of a HPLC feature map of a golden cypress medicinal material. The construction method comprises the following steps: reference solution preparation: preparing a golden cypress control medicinal material reference solution and a berberine hydrochloride and phellodendrine hydrochloride reference substance solution; sample solutionpreparation: preparing a golden cypress medicinal material sample solution and a decoction sample solution; high-performance liquid chromatography test: absorbing the control medicinal material reference solution, the reference substance solution, the medicinal material sample solution and the decoction sample solution; injecting into a liquid chromatograph, comparing a medicinal material samplemap with a decoction sample map, determining water soluble common peaks and obtaining the UPLC feature map of the golden cypress medicinal material. The HPLC feature map obtained by the construction method not only can qualita