Fluorescence quantitative reference genes suitable for fruits of clerodendrum trichotomum Thunb. of different provenances, primers and application of fluorescence quantitative internal reference genes
The invention discloses fluorescence quantitative reference genes suitable for fruits of clerodendrum trichotomum Thunb. of different provenances, primers and application of the fluorescence quantitative internal reference genes. The reference genes are a 18S gene and an EF-1A gene, wherein the gene...
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| creator | WANG LIANGGUI YUE YUANZHENG YANG XIULIAN HUA YAJIE GU HENG SHI TINGTING |
| description | The invention discloses fluorescence quantitative reference genes suitable for fruits of clerodendrum trichotomum Thunb. of different provenances, primers and application of the fluorescence quantitative internal reference genes. The reference genes are a 18S gene and an EF-1A gene, wherein the gene sequence of the 18S gene is shown in SEQ ID NO. 5, and the gene sequence of the EF-1A gene is shownin SEQ ID NO. 11. Based on existing disclosed genes, candidate genes with stable expression are screened, the stability of the candidate genes is evaluated through software, thus the reference genessuitable for the fruits of the clerodendrum trichotomum Thunb. of the different provenances and expressed most stably are obtained, and the real-time fluorescence quantitative PCR primers are designedbased on the genes; and reference genes under the different stages of the fruits of the clerodendrum trichotomum Thunb. of the different provenances appear for the first time, strong support is provided for accurate quantifica |
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The reference genes are a 18S gene and an EF-1A gene, wherein the gene sequence of the 18S gene is shown in SEQ ID NO. 5, and the gene sequence of the EF-1A gene is shownin SEQ ID NO. 11. Based on existing disclosed genes, candidate genes with stable expression are screened, the stability of the candidate genes is evaluated through software, thus the reference genessuitable for the fruits of the clerodendrum trichotomum Thunb. of the different provenances and expressed most stably are obtained, and the real-time fluorescence quantitative PCR primers are designedbased on the genes; and reference genes under the different stages of the fruits of the clerodendrum trichotomum Thunb. of the different provenances appear for the first time, strong support is provided for accurate quantifica</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190222&DB=EPODOC&CC=CN&NR=109371156A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25562,76317</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190222&DB=EPODOC&CC=CN&NR=109371156A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG LIANGGUI</creatorcontrib><creatorcontrib>YUE YUANZHENG</creatorcontrib><creatorcontrib>YANG XIULIAN</creatorcontrib><creatorcontrib>HUA YAJIE</creatorcontrib><creatorcontrib>GU HENG</creatorcontrib><creatorcontrib>SHI TINGTING</creatorcontrib><title>Fluorescence quantitative reference genes suitable for fruits of clerodendrum trichotomum Thunb. of different provenances, primers and application of fluorescence quantitative internal reference genes</title><description>The invention discloses fluorescence quantitative reference genes suitable for fruits of clerodendrum trichotomum Thunb. of different provenances, primers and application of the fluorescence quantitative internal reference genes. The reference genes are a 18S gene and an EF-1A gene, wherein the gene sequence of the 18S gene is shown in SEQ ID NO. 5, and the gene sequence of the EF-1A gene is shownin SEQ ID NO. 11. Based on existing disclosed genes, candidate genes with stable expression are screened, the stability of the candidate genes is evaluated through software, thus the reference genessuitable for the fruits of the clerodendrum trichotomum Thunb. of the different provenances and expressed most stably are obtained, and the real-time fluorescence quantitative PCR primers are designedbased on the genes; and reference genes under the different stages of the fruits of the clerodendrum trichotomum Thunb. of the different provenances appear for the first time, strong support is provided for accurate quantifica</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjrGOAjEMRLe5AnH8g-nhxAoBokTo0FVX0aOQnUCkrJ1zEr6Rz7qAqJCQqOyxx88zaK67UESRLNiC_orh7LPJ_gJSOOh9fAIjUSp1cwwgJ0pOq0okjmyASgfutPSU1duzZOlrvz8XPn7dLJ13d1SmqHIBmwpNkyp8D01kuCMTY_C2Pha-XbiXqTxnKJvwHO-z-XAmJIweddiMd9_77c8UUQ5I0VQW8mH7287W81XbLpab-Tuef7QVZmQ</recordid><startdate>20190222</startdate><enddate>20190222</enddate><creator>WANG LIANGGUI</creator><creator>YUE YUANZHENG</creator><creator>YANG XIULIAN</creator><creator>HUA YAJIE</creator><creator>GU HENG</creator><creator>SHI TINGTING</creator><scope>EVB</scope></search><sort><creationdate>20190222</creationdate><title>Fluorescence quantitative reference genes suitable for fruits of clerodendrum trichotomum Thunb. of different provenances, primers and application of fluorescence quantitative internal reference genes</title><author>WANG LIANGGUI ; YUE YUANZHENG ; YANG XIULIAN ; HUA YAJIE ; GU HENG ; SHI TINGTING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN109371156A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG LIANGGUI</creatorcontrib><creatorcontrib>YUE YUANZHENG</creatorcontrib><creatorcontrib>YANG XIULIAN</creatorcontrib><creatorcontrib>HUA YAJIE</creatorcontrib><creatorcontrib>GU HENG</creatorcontrib><creatorcontrib>SHI TINGTING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG LIANGGUI</au><au>YUE YUANZHENG</au><au>YANG XIULIAN</au><au>HUA YAJIE</au><au>GU HENG</au><au>SHI TINGTING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Fluorescence quantitative reference genes suitable for fruits of clerodendrum trichotomum Thunb. of different provenances, primers and application of fluorescence quantitative internal reference genes</title><date>2019-02-22</date><risdate>2019</risdate><abstract>The invention discloses fluorescence quantitative reference genes suitable for fruits of clerodendrum trichotomum Thunb. of different provenances, primers and application of the fluorescence quantitative internal reference genes. The reference genes are a 18S gene and an EF-1A gene, wherein the gene sequence of the 18S gene is shown in SEQ ID NO. 5, and the gene sequence of the EF-1A gene is shownin SEQ ID NO. 11. Based on existing disclosed genes, candidate genes with stable expression are screened, the stability of the candidate genes is evaluated through software, thus the reference genessuitable for the fruits of the clerodendrum trichotomum Thunb. of the different provenances and expressed most stably are obtained, and the real-time fluorescence quantitative PCR primers are designedbased on the genes; and reference genes under the different stages of the fruits of the clerodendrum trichotomum Thunb. of the different provenances appear for the first time, strong support is provided for accurate quantifica</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Fluorescence quantitative reference genes suitable for fruits of clerodendrum trichotomum Thunb. of different provenances, primers and application of fluorescence quantitative internal reference genes |
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