Method and specific primer pairs for identifying Salmo salar and onchorynchus mykiss
The invention discloses a method and specific primer pairs for identifying Salmo salar and onchorynchus mykiss. A Salmo salar primer pair is composed of a primer SS-5' and a primer SS-3', an onchorynchus mykiss primer pair is composed of a primer OM-5' and a primer OM-3', the pri...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method and specific primer pairs for identifying Salmo salar and onchorynchus mykiss. A Salmo salar primer pair is composed of a primer SS-5' and a primer SS-3', an onchorynchus mykiss primer pair is composed of a primer OM-5' and a primer OM-3', the primer SS-5', the primer SS-3', the primer OM-5' and the primer OM-3' are all single-stranded DNA molecules, and nucleotidesequences are a sequence 1 to a sequence 4 in a sequence table sequentially; the specific primer pair of the Salmo salar and/or the onchorynchus mykiss is adopted to conduct PCR amplification on genomic DNA of a to-be-detected sample, and whether the to-be-detected sample contains the Salmo salar and/or the onchorynchus mykiss or contains the Salmo salar and/or the onchorynchus mykiss in a candidate mode or not is judged according to whether effective amplification and DNA fragments in an amplification product are achieved or not. The specific primer pairs can be used in a mixed mode, can also be used individually an |
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