Poplar fungal ulcer bacteria ring mediated isothermal amplification rapid detection method

The invention discloses a poplar fungal ulcer bacteria ring mediated isothermal amplification rapid detection method. According to the method, an adopted primer group is composed of F3, B3, FIP and BIP, wherein F3 is GCCTGTTCGAGCGTCATT, B2 is TCAGCGGGTATCCCTACC, FIP is CGCTTGAGGCAAGACGCCACGCTCTGCTTG...

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Bibliographische Detailangaben
Hauptverfasser:	LU HONGYAN, HE BANGLING, WANG YILIN, WANG HAIMING, YU CHENGMING, LIU HUIXIANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a poplar fungal ulcer bacteria ring mediated isothermal amplification rapid detection method. According to the method, an adopted primer group is composed of F3, B3, FIP and BIP, wherein F3 is GCCTGTTCGAGCGTCATT, B2 is TCAGCGGGTATCCCTACC, FIP is CGCTTGAGGCAAGACGCCACGCTCTGCTTGGTATTGGGC, and BIP is ACATACATCTCGCTTCGGAGCGTCCGAGGTCAACCTTGAGAA. The detection method is simple andeffective, the detection efficiency is greatly improved, and the detection cost is lowered. 本发明公开了种杨树真菌性溃疡病菌环介导等温扩增快速检测方法，该方法采用的引物组由F3、B3、FIP、BIP组成，F3：GCCTGTTCGAGCGTCATT，B3：TCAGCGGGTATCCCTACC，FIP：CGCTTGAGGCAAGACGCCACGCTCTGCTTGGTATTGGGC，BIP：ACATACATCTCGCTTCGGAGCGTCCGAGGTCAACCTTGAGAA。本发明的检测方法简便有效，极大地提高了检测效率，降低了检测成本。