Compound amplification system and kit for MSI (microsatellite instability) gene mutation detection

The invention belongs to the field of biotechnology and particularly relates to a compound amplification system and a kit for MSI (microsatellite instability) gene mutation detection. The compound amplification system for MSI gene mutation detection is provided and amplifies mononucleotide microsatellite gene sites and dinucleotide microsatellite gene sites simultaneously; the mononucleotide microsatellite gene sites comprise NR-21, NR-22, NR-24, NR-27, BAT-25, BAT-26 and MONO-27; the dinucleotide microsatellite gene sites comprise one or more of D2S123, D17S250, D18S34 and D5S346. The mononucleotide microsatellite sites and the dinucleotide microsatellite sites are combined for detection and analysis of MSI, the detection accuracy and sensitivity can be effectively improved, and the system can be used for quickly detecting typing of tumor to guide treatment. 本发明属于生物技术领域，尤其涉及种微卫星不稳定性基因突变检测的复合扩增体系和试剂盒。本发明提供了种微卫星不稳定性基因突变检测的复合扩增体系，复合扩增体系同时对单核苷酸微卫星基因位点和二核苷酸微卫星基因位点进行扩增；所述单核苷酸微卫星基因位点包括NR-21、NR-22、NR-24、NR-27、BAT-25