Kit for detecting human matrix metalloproteinase 11 by one-step real-time fluorescence quantitative reverse transcription polymerase chain reaction
The invention relates to a kit for detecting human matrix metalloproteinase 11 by real-time fluorescence quantitative RT-PCR (Reverse Transcription Polymerase Chain Reaction). The kit takes mRNA as atemplate to carry out reverse transcription and real-time fluorescence quantitative PCR in the same r...
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| creator | YANG PEIHAO DENG JIEFEI |
| description | The invention relates to a kit for detecting human matrix metalloproteinase 11 by real-time fluorescence quantitative RT-PCR (Reverse Transcription Polymerase Chain Reaction). The kit takes mRNA as atemplate to carry out reverse transcription and real-time fluorescence quantitative PCR in the same reaction system without opening a pipe cover in a reaction process, so that cross-contamination is avoided, and the mRNA expression of the human matrix metalloproteinase 11 (MMP11) in a specimen can be accurately and quantitatively detected. The kit can be used for clinical and scientific researchesto detect the expression of the MMP11 in a surgically resected specimen of a colorectal cancer of a patient, so as to realize auxiliary diagnosis, guiding treatment and prognosis for colorectal cancer patients.
本发明为种实时荧光定量RT-PCR检测人基质金属蛋白酶11的试剂盒，该试剂盒以mRNA为模板，使反转录和实时荧光定量PCR在同反应体系中进行，反应过程中无需打开管盖，避免了交叉污染，并可以精确定量检测标本中人基质金属蛋白酶11(MMP11)的mRNA表达量。该试剂盒可以用于临床及科研中检测患者结直肠癌手术切除标本中MMP11的表达，用以结直肠癌患者的辅助诊断、指导治疗及提示预后。 |
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本发明为种实时荧光定量RT-PCR检测人基质金属蛋白酶11的试剂盒，该试剂盒以mRNA为模板，使反转录和实时荧光定量PCR在同反应体系中进行，反应过程中无需打开管盖，避免了交叉污染，并可以精确定量检测标本中人基质金属蛋白酶11(MMP11)的mRNA表达量。该试剂盒可以用于临床及科研中检测患者结直肠癌手术切除标本中MMP11的表达，用以结直肠癌患者的辅助诊断、指导治疗及提示预后。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190215&DB=EPODOC&CC=CN&NR=109337961A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190215&DB=EPODOC&CC=CN&NR=109337961A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YANG PEIHAO</creatorcontrib><creatorcontrib>DENG JIEFEI</creatorcontrib><title>Kit for detecting human matrix metalloproteinase 11 by one-step real-time fluorescence quantitative reverse transcription polymerase chain reaction</title><description>The invention relates to a kit for detecting human matrix metalloproteinase 11 by real-time fluorescence quantitative RT-PCR (Reverse Transcription Polymerase Chain Reaction). The kit takes mRNA as atemplate to carry out reverse transcription and real-time fluorescence quantitative PCR in the same reaction system without opening a pipe cover in a reaction process, so that cross-contamination is avoided, and the mRNA expression of the human matrix metalloproteinase 11 (MMP11) in a specimen can be accurately and quantitatively detected. The kit can be used for clinical and scientific researchesto detect the expression of the MMP11 in a surgically resected specimen of a colorectal cancer of a patient, so as to realize auxiliary diagnosis, guiding treatment and prognosis for colorectal cancer patients.
本发明为种实时荧光定量RT-PCR检测人基质金属蛋白酶11的试剂盒，该试剂盒以mRNA为模板，使反转录和实时荧光定量PCR在同反应体系中进行，反应过程中无需打开管盖，避免了交叉污染，并可以精确定量检测标本中人基质金属蛋白酶11(MMP11)的mRNA表达量。该试剂盒可以用于临床及科研中检测患者结直肠癌手术切除标本中MMP11的表达，用以结直肠癌患者的辅助诊断、指导治疗及提示预后。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjUFqAkEQRWfjImjuUDnAgMNAgkuRiCBklb1UOn9iQXd1W10j8Ry5sDPgAbL6i__--0_N31Gchmz0DUdw0R86j4mVErvJLyU4x5iLZYcoV1DX0deNsqKtjkIGjq1LAg1xzIYaoAF0GVldnF2umJgrbJq6sdZgUlyyUsnxlmCzM5xZdFaFuVk1i4FjxfMjl83L_v1zd2hR8gm18HQBP-0-uvWm7982r922_w9zByWTUa4</recordid><startdate>20190215</startdate><enddate>20190215</enddate><creator>YANG PEIHAO</creator><creator>DENG JIEFEI</creator><scope>EVB</scope></search><sort><creationdate>20190215</creationdate><title>Kit for detecting human matrix metalloproteinase 11 by one-step real-time fluorescence quantitative reverse transcription polymerase chain reaction</title><author>YANG PEIHAO ; DENG JIEFEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN109337961A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YANG PEIHAO</creatorcontrib><creatorcontrib>DENG JIEFEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YANG PEIHAO</au><au>DENG JIEFEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Kit for detecting human matrix metalloproteinase 11 by one-step real-time fluorescence quantitative reverse transcription polymerase chain reaction</title><date>2019-02-15</date><risdate>2019</risdate><abstract>The invention relates to a kit for detecting human matrix metalloproteinase 11 by real-time fluorescence quantitative RT-PCR (Reverse Transcription Polymerase Chain Reaction). The kit takes mRNA as atemplate to carry out reverse transcription and real-time fluorescence quantitative PCR in the same reaction system without opening a pipe cover in a reaction process, so that cross-contamination is avoided, and the mRNA expression of the human matrix metalloproteinase 11 (MMP11) in a specimen can be accurately and quantitatively detected. The kit can be used for clinical and scientific researchesto detect the expression of the MMP11 in a surgically resected specimen of a colorectal cancer of a patient, so as to realize auxiliary diagnosis, guiding treatment and prognosis for colorectal cancer patients.
本发明为种实时荧光定量RT-PCR检测人基质金属蛋白酶11的试剂盒，该试剂盒以mRNA为模板，使反转录和实时荧光定量PCR在同反应体系中进行，反应过程中无需打开管盖，避免了交叉污染，并可以精确定量检测标本中人基质金属蛋白酶11(MMP11)的mRNA表达量。该试剂盒可以用于临床及科研中检测患者结直肠癌手术切除标本中MMP11的表达，用以结直肠癌患者的辅助诊断、指导治疗及提示预后。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Kit for detecting human matrix metalloproteinase 11 by one-step real-time fluorescence quantitative reverse transcription polymerase chain reaction |
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