Kit for detecting human matrix metalloproteinase 11 by one-step real-time fluorescence quantitative reverse transcription polymerase chain reaction

The invention relates to a kit for detecting human matrix metalloproteinase 11 by real-time fluorescence quantitative RT-PCR (Reverse Transcription Polymerase Chain Reaction). The kit takes mRNA as atemplate to carry out reverse transcription and real-time fluorescence quantitative PCR in the same reaction system without opening a pipe cover in a reaction process, so that cross-contamination is avoided, and the mRNA expression of the human matrix metalloproteinase 11 (MMP11) in a specimen can be accurately and quantitatively detected. The kit can be used for clinical and scientific researchesto detect the expression of the MMP11 in a surgically resected specimen of a colorectal cancer of a patient, so as to realize auxiliary diagnosis, guiding treatment and prognosis for colorectal cancer patients. 本发明为种实时荧光定量RT-PCR检测人基质金属蛋白酶11的试剂盒，该试剂盒以mRNA为模板，使反转录和实时荧光定量PCR在同反应体系中进行，反应过程中无需打开管盖，避免了交叉污染，并可以精确定量检测标本中人基质金属蛋白酶11(MMP11)的mRNA表达量。该试剂盒可以用于临床及科研中检测患者结直肠癌手术切除标本中MMP11的表达，用以结直肠癌患者的辅助诊断、指导治疗及提示预后。