Short hairpin shRNA capable of suppressing expression of ILT5, lentivirus and application of short hairpin shRNA or lentivirus

The invention discloses a short hairpin shRNA capable of suppressing the expression of ILT5. The nucleotide sequence of the short hairpin shRNA is shown in SEQ ID NO. 1 or SEQ ID NO. 2. The inventionfurther discloses an ILT5 gene shRNA knock-down lentivirus expression vector obtained by inserting th...

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Hauptverfasser: SHI HONG'EN, JIANG FENGLIANG, GAO XINGCHUN, MI YAJING, SU XINGLI, GOU XINGCHUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a short hairpin shRNA capable of suppressing the expression of ILT5. The nucleotide sequence of the short hairpin shRNA is shown in SEQ ID NO. 1 or SEQ ID NO. 2. The inventionfurther discloses an ILT5 gene shRNA knock-down lentivirus expression vector obtained by inserting the short hairpin shRNA capable of suppressing the expression of ILT5 into a lentivirus vector plasmid pGLVH3/H1/GFP+Puro, a preparation method of an ILT5 virus, and application of the short hairpin shRNA capable of suppressing the expression of ILT5, or the lentivirus expression vector, or the ILT5virus to preparing medicines for inhibiting proliferation, migration and invasion of glioma, and proliferation of glioma stem cells. 本发明公开了种抑制ILT5表达的短发卡shRNA,其核苷酸序列如SEQ ID No.1或SEQ ID No.2所示;本发明还公开了种ILT5基因shRNA敲低慢病毒表达载体,将抑制ILT5表达的短发卡shRNA插入慢病毒载体质粒pGLVH3/H1/GFP+Puro所得;以及种ILT5病毒的制备方法制备得到ILT5病毒;本发明还公开了种ILT5病毒的制备方法以及抑制ILT5表达的短发卡shRNA或慢病毒表达载体或ILT5病毒在制备抑制胶质瘤增殖、抑制胶质瘤迁移、抑制胶质瘤侵袭、抑制胶质瘤干细胞增殖的药物中的应用。