Method for enucleating mammal oocyte
The invention belongs to the technical field of embryo engineering, and particularly relates to a method for enucleating mammal oocyte. The method comprises the following steps: obtaining cumulus oocyte compositelex; removing cumulus granulose cells at the periphery of an oocyte transparent zone in...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | MAI RANBIAO WU ZHENFANG CAI GENGYUAN ZHOU RONG YU WANXIAN LUO LYUHUA SHI JUNSONG |
| description | The invention belongs to the technical field of embryo engineering, and particularly relates to a method for enucleating mammal oocyte. The method comprises the following steps: obtaining cumulus oocyte compositelex; removing cumulus granulose cells at the periphery of an oocyte transparent zone in the cumulus oocyte compositelex, and leaving oocyte; incubating the obtained oocyte to nucleate; putting the nucleated oocyte into culture solution liquor to treat; debugging a fluorescent lamp; cleaning the oocyte treated by the culture solutionliquor, and transferring the cleaned oocyte into enucleatingstoning liquid drops; and enucleating under fluorescence. The method is simple and convenient to operate, greatly reduces loss of cytoplasm, ensures that each constructed clone embryo loses a little cytoplasm and achieves 100% enucleating, and improves developmental potentiality of the embryos.
本发明属于胚胎工程技术领域，具体涉及种哺乳动物卵母细胞去核的方法。本发明方法包括以下步骤：获取卵丘卵母细胞复合体；去除卵丘卵母细胞复合体中卵母细胞透明带外围的卵丘颗粒细胞，剩下卵母细胞；将所得的卵母细胞孵育显核；将显核后的卵母细胞置于培养液中处 |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN109321518A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN109321518A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN109321518A3</originalsourceid><addsrcrecordid>eNrjZFDxTS3JyE9RSMsvUkjNK03OSU0sycxLV8hNzM1NzFHIz0-uLEnlYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GBpbGRoamhhaOxsSoAQAN_ibG</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for enucleating mammal oocyte</title><source>esp@cenet</source><creator>MAI RANBIAO ; WU ZHENFANG ; CAI GENGYUAN ; ZHOU RONG ; YU WANXIAN ; LUO LYUHUA ; SHI JUNSONG</creator><creatorcontrib>MAI RANBIAO ; WU ZHENFANG ; CAI GENGYUAN ; ZHOU RONG ; YU WANXIAN ; LUO LYUHUA ; SHI JUNSONG</creatorcontrib><description>The invention belongs to the technical field of embryo engineering, and particularly relates to a method for enucleating mammal oocyte. The method comprises the following steps: obtaining cumulus oocyte compositelex; removing cumulus granulose cells at the periphery of an oocyte transparent zone in the cumulus oocyte compositelex, and leaving oocyte; incubating the obtained oocyte to nucleate; putting the nucleated oocyte into culture solution liquor to treat; debugging a fluorescent lamp; cleaning the oocyte treated by the culture solutionliquor, and transferring the cleaned oocyte into enucleatingstoning liquid drops; and enucleating under fluorescence. The method is simple and convenient to operate, greatly reduces loss of cytoplasm, ensures that each constructed clone embryo loses a little cytoplasm and achieves 100% enucleating, and improves developmental potentiality of the embryos.
本发明属于胚胎工程技术领域，具体涉及种哺乳动物卵母细胞去核的方法。本发明方法包括以下步骤：获取卵丘卵母细胞复合体；去除卵丘卵母细胞复合体中卵母细胞透明带外围的卵丘颗粒细胞，剩下卵母细胞；将所得的卵母细胞孵育显核；将显核后的卵母细胞置于培养液中处</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190212&DB=EPODOC&CC=CN&NR=109321518A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190212&DB=EPODOC&CC=CN&NR=109321518A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MAI RANBIAO</creatorcontrib><creatorcontrib>WU ZHENFANG</creatorcontrib><creatorcontrib>CAI GENGYUAN</creatorcontrib><creatorcontrib>ZHOU RONG</creatorcontrib><creatorcontrib>YU WANXIAN</creatorcontrib><creatorcontrib>LUO LYUHUA</creatorcontrib><creatorcontrib>SHI JUNSONG</creatorcontrib><title>Method for enucleating mammal oocyte</title><description>The invention belongs to the technical field of embryo engineering, and particularly relates to a method for enucleating mammal oocyte. The method comprises the following steps: obtaining cumulus oocyte compositelex; removing cumulus granulose cells at the periphery of an oocyte transparent zone in the cumulus oocyte compositelex, and leaving oocyte; incubating the obtained oocyte to nucleate; putting the nucleated oocyte into culture solution liquor to treat; debugging a fluorescent lamp; cleaning the oocyte treated by the culture solutionliquor, and transferring the cleaned oocyte into enucleatingstoning liquid drops; and enucleating under fluorescence. The method is simple and convenient to operate, greatly reduces loss of cytoplasm, ensures that each constructed clone embryo loses a little cytoplasm and achieves 100% enucleating, and improves developmental potentiality of the embryos.
本发明属于胚胎工程技术领域，具体涉及种哺乳动物卵母细胞去核的方法。本发明方法包括以下步骤：获取卵丘卵母细胞复合体；去除卵丘卵母细胞复合体中卵母细胞透明带外围的卵丘颗粒细胞，剩下卵母细胞；将所得的卵母细胞孵育显核；将显核后的卵母细胞置于培养液中处</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZFDxTS3JyE9RSMsvUkjNK03OSU0sycxLV8hNzM1NzFHIz0-uLEnlYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GBpbGRoamhhaOxsSoAQAN_ibG</recordid><startdate>20190212</startdate><enddate>20190212</enddate><creator>MAI RANBIAO</creator><creator>WU ZHENFANG</creator><creator>CAI GENGYUAN</creator><creator>ZHOU RONG</creator><creator>YU WANXIAN</creator><creator>LUO LYUHUA</creator><creator>SHI JUNSONG</creator><scope>EVB</scope></search><sort><creationdate>20190212</creationdate><title>Method for enucleating mammal oocyte</title><author>MAI RANBIAO ; WU ZHENFANG ; CAI GENGYUAN ; ZHOU RONG ; YU WANXIAN ; LUO LYUHUA ; SHI JUNSONG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN109321518A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>MAI RANBIAO</creatorcontrib><creatorcontrib>WU ZHENFANG</creatorcontrib><creatorcontrib>CAI GENGYUAN</creatorcontrib><creatorcontrib>ZHOU RONG</creatorcontrib><creatorcontrib>YU WANXIAN</creatorcontrib><creatorcontrib>LUO LYUHUA</creatorcontrib><creatorcontrib>SHI JUNSONG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MAI RANBIAO</au><au>WU ZHENFANG</au><au>CAI GENGYUAN</au><au>ZHOU RONG</au><au>YU WANXIAN</au><au>LUO LYUHUA</au><au>SHI JUNSONG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for enucleating mammal oocyte</title><date>2019-02-12</date><risdate>2019</risdate><abstract>The invention belongs to the technical field of embryo engineering, and particularly relates to a method for enucleating mammal oocyte. The method comprises the following steps: obtaining cumulus oocyte compositelex; removing cumulus granulose cells at the periphery of an oocyte transparent zone in the cumulus oocyte compositelex, and leaving oocyte; incubating the obtained oocyte to nucleate; putting the nucleated oocyte into culture solution liquor to treat; debugging a fluorescent lamp; cleaning the oocyte treated by the culture solutionliquor, and transferring the cleaned oocyte into enucleatingstoning liquid drops; and enucleating under fluorescence. The method is simple and convenient to operate, greatly reduces loss of cytoplasm, ensures that each constructed clone embryo loses a little cytoplasm and achieves 100% enucleating, and improves developmental potentiality of the embryos.
本发明属于胚胎工程技术领域，具体涉及种哺乳动物卵母细胞去核的方法。本发明方法包括以下步骤：获取卵丘卵母细胞复合体；去除卵丘卵母细胞复合体中卵母细胞透明带外围的卵丘颗粒细胞，剩下卵母细胞；将所得的卵母细胞孵育显核；将显核后的卵母细胞置于培养液中处</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN109321518A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for enucleating mammal oocyte |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T01%3A35%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=MAI%20RANBIAO&rft.date=2019-02-12&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN109321518A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |