Method for enucleating mammal oocyte

The invention belongs to the technical field of embryo engineering, and particularly relates to a method for enucleating mammal oocyte. The method comprises the following steps: obtaining cumulus oocyte compositelex; removing cumulus granulose cells at the periphery of an oocyte transparent zone in the cumulus oocyte compositelex, and leaving oocyte; incubating the obtained oocyte to nucleate; putting the nucleated oocyte into culture solution liquor to treat; debugging a fluorescent lamp; cleaning the oocyte treated by the culture solutionliquor, and transferring the cleaned oocyte into enucleatingstoning liquid drops; and enucleating under fluorescence. The method is simple and convenient to operate, greatly reduces loss of cytoplasm, ensures that each constructed clone embryo loses a little cytoplasm and achieves 100% enucleating, and improves developmental potentiality of the embryos. 本发明属于胚胎工程技术领域，具体涉及种哺乳动物卵母细胞去核的方法。本发明方法包括以下步骤：获取卵丘卵母细胞复合体；去除卵丘卵母细胞复合体中卵母细胞透明带外围的卵丘颗粒细胞，剩下卵母细胞；将所得的卵母细胞孵育显核；将显核后的卵母细胞置于培养液中处