Primers and method for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha and application of primers

The invention discloses primers and method for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha. The primers include an upstream primer (shown as SEQ ID NO: 1) and a downward primer (shown as SEQ ID NO: 2). The specific primers are designed according to regional characteristics of genetic polymorphism; a DNA fragment containing a conserved region and a polymorphic region is acquired by PCR (polymerase chain reaction); whether PvMSP-3alpha gene mutates can be identified through sequencing and analysis; the gene type being wild or mutant can be distinguished then. In addition, the invention also discloses application of the specific primers for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha in the preparation of detection tools for identifying genetic polymorphism of Plasmodium vivax PvMSP-3alpha. 本发明公开了种鉴定间日疟原虫PvMSP-3α基因多态性的引物及方法，包括上游引物（如SEQ ID NO：1所示）和下游引物（如SEQ ID NO：2所示）。根据基因多态性区域特征，设计特定的引物，经PCR扩增得到包含保守区域和多态性区域的DNA片段，通过测序、分析，能够对PvMSP-3α基因是否突变进行鉴定，进而区分出是野生型还是突变型。此外，本发明还公开了该