Vaccine strain rSN-R92G-E93K, construction method and applications thereof

The invention provides a vaccine strain rSN-R92G-E93K, and belongs to the technical field of influenza vaccine preparation. The construction method comprises: preparing an expression vector pHW-SN-HAfrom a HA gene fragment; carrying out point mutation on the expression vector; co-transforming cells...

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Hauptverfasser:	LIU LEI, CHEN SUJUAN, QIN TAO, WANG ZEYUAN, LI ZHUOTIAN, ZHANG WEI, QIAO YIYI, SHI BAOLAN, SU XIANG, PENG DAXIN, SHEN HAIFENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a vaccine strain rSN-R92G-E93K, and belongs to the technical field of influenza vaccine preparation. The construction method comprises: preparing an expression vector pHW-SN-HAfrom a HA gene fragment; carrying out point mutation on the expression vector; co-transforming cells by using the obtained expression plasmid SNHA-R92G-E93K and plasmids containing other genes; and carrying out recombination. According to the present invention, the vaccine strain rSN-R92G-E93K has the HI titer of 11.6+/-0.5 log2 and the serum neutralizing titer of 296.50+/-103.95 log2, such that the vaccine strain rSN-R92G-E93K is an ideal vaccine strain. 本发明提供了株疫苗株rSN-R92G-E93K，属于流感疫苗制备技术领域，将HA基因片段制备成表达载体pHW-SN-HA，再对表达载体进行点突变，将得到的表达质粒SNHA-R92G-E93K再与含有其它基因质粒共转染细胞、重组后得到。本发明提供的疫苗株rSN-R92G-E93K的HI效价为11.6±0.5log2，血清中和效价为296.50±103.95log2，由此能够得出疫苗株rSN-R92G-E93K是株比较理想的疫苗株。