Method for detecting endotoxin

The invention discloses a method for detecting endotoxin. The method comprises the following steps of performing incubation on a to-be-detected sample and a fluorescently-labeled LPS aptamer; then adding reduced graphene oxide and a TBE buffer solution, and performing incubation; adding the incubate...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: LIU LIHONG, NIU JUNXIN
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention discloses a method for detecting endotoxin. The method comprises the following steps of performing incubation on a to-be-detected sample and a fluorescently-labeled LPS aptamer; then adding reduced graphene oxide and a TBE buffer solution, and performing incubation; adding the incubated sample into a sample inlet of a micro-fluidic chip, applying a voltage between the sample inlet and a sample outlet of the micro-fluidic chip to perform processing, arranging an anode at the sample inlet, and detecting the fluorescence intensity between a Nafion membrane channel and the anode after the voltage processing; and determining the content of LPS in the to-be-detected sample according to the fluorescence intensity and a standard curve. The method has a lowest detection limit of 8 fM,is wide in linear range, high in sensitivity, good in selectivity and relatively strong in anti-interference capability, and can rapidly distinguish gram-positive bacteria, gram-negative bacteria andfungi. 本发明公开了种检测内毒素的方法,该方