Method for detecting endotoxin
The invention discloses a method for detecting endotoxin. The method comprises the following steps of performing incubation on a to-be-detected sample and a fluorescently-labeled LPS aptamer; then adding reduced graphene oxide and a TBE buffer solution, and performing incubation; adding the incubate...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for detecting endotoxin. The method comprises the following steps of performing incubation on a to-be-detected sample and a fluorescently-labeled LPS aptamer; then adding reduced graphene oxide and a TBE buffer solution, and performing incubation; adding the incubated sample into a sample inlet of a micro-fluidic chip, applying a voltage between the sample inlet and a sample outlet of the micro-fluidic chip to perform processing, arranging an anode at the sample inlet, and detecting the fluorescence intensity between a Nafion membrane channel and the anode after the voltage processing; and determining the content of LPS in the to-be-detected sample according to the fluorescence intensity and a standard curve. The method has a lowest detection limit of 8 fM,is wide in linear range, high in sensitivity, good in selectivity and relatively strong in anti-interference capability, and can rapidly distinguish gram-positive bacteria, gram-negative bacteria andfungi.
本发明公开了种检测内毒素的方法,该方 |
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