Detection method of detecting negative ions and positive ions at same time

The invention provides a method of detecting negative ions and positive ions at the same time. A high performance liquid chromatography is used for combining an electric spraying detector; a chromatographic column is an SeQuant ZIC pHILIC ion chromatographic column; a flow phase A is a water solution of 25mM ammonium acetate; a mobile phase B is acetonitrile; a mobile phase C is methyl alcohol; anHLLIC mode and a gradient elution mode are used; almost all common negative ions and positive ions can be detected; the separation effect is good; the separation degree reaches 1.5 or higher; the response to each ion is high; the detection limit of all ions is low. 本发明提供种同时检测阴阳离子的方法，采用高效液相色谱联合电喷雾检测器，色谱柱为SeQuant ZIC pHILIC离子色谱柱，流动相A为25mM醋酸铵的水溶液，流动相B为乙腈，流动相C为甲醇，采用HILIC模式和梯度洗脱模式，能够检测出几乎所有的常见阴离子和阳离子，且分离效果好，分离度达1.5以上，对各个离子响应高，各个离子的检测限低。