Method for preparing omega-3 fatty acid-rich lysophospholipid by one-step enzymatic method

The invention discloses a method for preparing omega-3 fatty acid-rich lysophospholipid by one-step enzymatic method. Phospholipid is dissolved in ethanol, lipase and water are added, alcoholysis is carried out to obtain omega-3 Lysophospholipid of fatty acids, the lipase is added in an amount of 1 to 20% by weight of phospholipid and 3 to 15% by weight of water, and the lipase is selected from one or more of Novozyme@TL 100, Novozyme@40116 and Lipozyme@RM. The yield of lysophospholipid is high, omega-3 fatty acid concentration is high, reaction cycle is short, separation and purification process is simple. In a second aspect of the present invention, the omega-3 enriched compound obtained by using the preparation method provided in the present invention provides a reference for the preparation of anticancer drugs. 本发明公开了种采用步酶法制备富含ω-3脂肪酸溶血磷脂的方法，将磷脂溶于乙醇中，添加脂肪酶和水，进行醇解，得到富含ω-3脂肪酸的溶血磷脂；其中，脂肪酶的添加量占磷脂重量的1～20%，水的添加量占磷脂和乙醇总重量的3%～15%，脂肪酶选自Novozyme@TL 100、Novozyme@40116、Lipozyme@RM中的种或两种以上。溶血磷脂的得率高，ω-3脂肪酸富集浓度高，反应周期短，分离