Spontaneously lighting kansas mycobacterium and construction method thereof

The invention discloses a spontaneously lighting kansas mycobacterium. The spontaneously lighting kansas mycobacterium is based on Mycobacterium Kansas, which is inserted into a recombinant plasmid pOPTIg by electroporation and screened by subculture. The autonomously illuminated Mycobacterium kansi...

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Bibliographische Detailangaben
Hauptverfasser:	WANG YUYE, HAO JIANBANG, FANG KUN, WANG BANGXING, LIU CHEN, CHENG JIE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a spontaneously lighting kansas mycobacterium. The spontaneously lighting kansas mycobacterium is based on Mycobacterium Kansas, which is inserted into a recombinant plasmid pOPTIg by electroporation and screened by subculture. The autonomously illuminated Mycobacterium kansii provided by the present invention is more stable in subculture; the method for constructing autonomously luminescent Kansas vaginalis designed by the present invention, in the process of providing a recombinant plasmid pOPTIg, the recombinant plasmid pOPTIg is transferred Screening in Mycobacterium kansii to obtain autonomously luminescent Kansas bacillus with no resistance screening markers for loss of resistance genes, integrase genes, and integration sites. 本发明公开了种自主发光堪萨斯分枝杆菌，该自主发光堪萨斯分枝杆菌基于堪萨斯分枝杆菌，采用电转化法，插入重组质粒pOPTIg，经传代培养筛选而成。本发明提供的自主发光堪萨斯分枝杆菌在传代培养中更加稳定；本发明设计的种自主发光堪萨斯分枝杆菌的构建方法，在该构建过程中，提供重组质粒pOPTIg，该重组质粒pOPTIg转入堪萨斯分枝杆菌中经过筛选，从而获得抗性基因、整合酶基因和整合位点均丢失的无抗性筛选标记的自主发光堪萨斯分枝杆菌。