Sterilization method of rape seeds for tissue culture

The invention discloses a sterilization method of rape seeds for tissue culture. The sterilization method comprises the following steps: firstly, putting seeds in water for socking for 1 hour, removing seeds floating on the water surface, washing with tap water twice, transferring into a clean bench, sterilizing for 1 minute with 75% alcohol, sterilizing the seeds for 15 minutes with 3% NaClO (keeping a shaking and rotating state in the process), further washing with sterile water 5-6 times, and finally transferring the seeds onto sterile filter paper for airing, wherein the 3% NaClO has the optimal sterilization concentration screened from seed sterilization effects of 0-30% NaClO, and the sterilization time of 15 minutes is the optimal sterilization time screened from 10-30 minutes of seed sterilization with the optimal concentration of 3% NaClO. By adopting the sterilization method, maximum healthy seedlings can be cultured for the production of transgenic oilseed rape, so that thesuccess rate of oilseed r