A molecular marker suitable for detecting purple radish and its application

The invention belongs to the technical field of molecular markers for vegetables, in particular to a molecular marker suitable for detecting the characteristics of purple radish and its application. The invention obtains F1 plant by selecting suitable parent hybridization. The F2 segregation population is obtained by self-bagging F1 plants after seeding the F1 seeds. By constructing purple radishgene pool and white radish gene pool, group analysis (BSA) and AFLP technique are used to screen the molecular marker RA-20, the nucleotide sequence of the molecular marker is shown as SEQ ID NO. 1, which can be used for population analysis of purple radish and provides practical markers and methods for molecular breeding of radish. 本发明属于蔬菜分子标记技术领域，具体涉及种适用于检测紫色萝卜性状的分子标记及应用。本发明通过选择合适的亲本杂交得到F植株，播种该F种子，将F植株套袋自交得到F分离群体，通过构建紫色萝卜基因池和白色萝卜基因池、集团分析法（BSA）与AFLP技术相结合筛选得到与萝卜紫色基因紧密连锁的分子标记RA-20，该分子标记的核苷酸序列如SEQ ID NO.1所示，可用于紫色萝卜群体分析，为萝卜分子育种提了实用标记和方法。